* Gnomeo: A blue gnome and the protagonist of the story. He is the son of Lady Bluebury and is in love with Juliet.
* Juliet: A red gnome and the daughter of Lord Redbrick. She is in love with Gnomeo.
* Tybalt: A blue gnome and the cousin of Gnomeo. He is hot-headed and impulsive, and often picks fights with the red gnomes.
* Lord Redbrick: The leader of the red gnomes. He is Juliet's father and does not approve of her relationship with Gnomeo.
* Lady Bluebury: The leader of the blue gnomes. She is Gnomeo's mother and is supportive of his relationship with Juliet.
* Benny: A blue gnome and Gnomeo's best friend. He is always there for Gnomeo and is a voice of reason.
* Chloe: A red gnome and Juliet's best friend. She is also a voice of reason for Juliet and is supportive of her relationship with Gnomeo.
* Paris: A red gnome who is in love with Juliet. He is not aware that Juliet is already in love with Gnomeo and tries to win her over.
* Featherstone: A flamingo who is the wise old sage of the garden. He offers advice and guidance to the gnomes.
* Miss Montague: A gnome who is the head of the garden gnomes' association. She is fair and just, and tries to keep the peace between the red and blue gnomes.