- Angela Lansbury as Mame Dennis
- Bea Arthur as Vera Charles
- Robert Preston as Beauregard Jackson Pickett Burnside
- Jane Connell as Agnes Gooch
- Frankie Michaels as Patrick Dennis (age 10)
- Will Geer as Babcock
- Charles Braswell as Mr. Upson
- Barbara Baxley as Mrs. Upson
- Cory Bevington as Pegeen Ryan
- John van Dreelen as Dwight Babcock