Television:
* The Rookie (2018 - present): Sergeant "Nyla" Harper
* The Wire (2002-2008): Detective "Beadie" Russell
* Six Feet Under (2001-2005): Brenda Chenowith
* ER (1994-2009): Dr. Janet Coburn
* The X-Files (1993-2018): Dr. Anne Buckle
* The West Wing (1999-2006): Dr. Grace Barnett
* The Good Wife (2009-2016): Judge Christine "Chrissy" Sexton
* Castle (2009-2016): Detective Ryan "Ritter" Russell
* Law & Order: Special Victims Unit (1999-present): Various roles
* Grey's Anatomy (2005-present): Dr. Beth Hunter
* NCIS (2003-present): Dr. Wendy Kieffer
* Criminal Minds (2005-2020): Dr. Erica Rinehart
* Modern Family (2009-2020): Nurse Pam
Film:
* The Sisterhood of the Traveling Pants (2005): Mrs. Gold
* The Perfect Score (2004): Mrs. Baker
* The Day After Tomorrow (2004): Dr. Lucy Hall
* The Prince of Tides (1991): Martha McMahon
* Thelma & Louise (1991): Carhop
* The Silence of the Lambs (1991): Woman at the Gas Station
* The Player (1992): Secretary
This is just a partial list, as Amy Aquino has been in many other projects throughout her career. She is a versatile actress with a strong presence on both the big and small screen.