The second apology occurs during their duel, which is also in Act 5 Scene 2. As the duel progresses, Hamlet once again apologizes to Laertes, recognizing the harm he caused to Laertes and his family. He expresses his genuine sorrow and regret for the pain he inflicted.
Throughout the apology, Hamlet emphasizes that his actions were not out of malice or any personal grudge, but rather due to the circumstances that unfolded around him. He acknowledges his flaws and takes responsibility for his deeds.