Mrs. Arable: Mr. Arable's wife is also a kind and hardworking woman. She helps her husband with the farm work and takes care of the animals.
Fern Arable: Fern is the daughter of Mr. and Mrs. Arable. She is a curious and imaginative young girl who loves animals. She is the one who finds Charlotte and saves her from being killed.
Avery Arable: Avery is Fern's younger brother. He is also curious and imaginative, but he is not as kind as Fern. He sometimes gets into trouble, but he always means well.
Henry Fussy: Mr. Fussy is the owner of the fair where Charlotte and Wilbur are exhibited. He is a greedy and selfish man who cares more about money than about the animals.
Dr. Dorian: Dr. Dorian is the veterinarian who helps to deliver Wilbur's piglets. He is a kind and compassionate man who cares about the animals he treats.
Goose: Goose is the goose who lives on the Arable farm. He is a proud and pompous bird who thinks himself better than other animals.
Templeton the Rat: Templeton is a sly and cunning rat who is always looking for a free meal. He is not a very nice animal, but he is sometimes helpful to Charlotte and Wilbur.