* "Steamboat Willie" (1928): This is often considered the first sound Disney film, and it's the one that launched Mickey Mouse into stardom.
* "Alice's Wonderland" (1923): This was Disney's first live-action/animated film series, featuring a live-action girl interacting with animated characters.
* "Plane Crazy" (1928): This was the first film to feature Mickey Mouse, but it was initially a silent film.
So, depending on your definition of "first," you could argue for any of these films.