Protagonists:
* Cinderella: The kind and gentle young woman who is mistreated by her stepmother and stepsisters.
* The Prince: A handsome and charming prince who is looking for a wife.
* The Fairy Godmother: A magical fairy who helps Cinderella get to the ball.
Antagonists:
* Lady Tremaine (The Stepmother): A cruel and controlling woman who makes Cinderella's life miserable.
* Drizella Tremaine: The stepmother's elder daughter, who is spoiled and nasty.
* Anastasia Tremaine: The stepmother's younger daughter, who is equally spoiled and nasty.
Supporting Characters:
* Gus and Jaq: Two mice who are Cinderella's loyal friends.
* Bruno: A dog who is Cinderella's loyal friend.
* The King: The Prince's father, who is kind and supportive.
* The Grand Duke: The King's advisor, who helps the Prince find a wife.
Other Notable Characters:
* The Duke's Attendant: The Grand Duke's assistant who helps him prepare for the ball.
* The Royal Guard: The King's soldiers who protect the castle.
There are also several unnamed animal characters and townsfolk who appear in the film.