Double click the desktop icon to launch Fruity Loops 9. It may take a few seconds for the program to fully launch.
Click "File" and select "Open." From here, select your preferred option. Either click on a work-in-progress session or select "New." If you select "New," you must then click "File" and select "Import" to import an audio file. Otherwise there will be nothing to use Autotune on.
Click "Mixer" tab. This opens the mixer window. Fruity Loops Studio 9 has two view options, window view and arrange view. Arrange is the view to use for editing in a linear fashion, for example if time-stretching or editing audio. The mixer view is for balancing audio and applying effects, such as Autotune.
Click "Menu" and select "Autotune." This opens Autotune in a separate window.
Adjust the "Retune Speed" parameter. This setting defines the intensity of the effect. For a subtle, corrective effect set it to anywhere between 50 and 80 percent. Set it to zero if you're seeking to create the "Cher Effect." This phenomenon is caused when Autotune instantly corrects a note so it jumps from one pitch to another with no gradient in between. The characteristic vocal sound on Cher's 1999 song "Believe" was created by using a zero retune speed setting.
Select a key. Where it says "Key" enter the key of the audio, for example "A Major." If you don't know the key, leave this option blank and Autotune will attempt to detect the key using its pitch-detection algorithm.
Click on "Input Type." Select the option that best matches the vocal range of the audio, for example "Soprano" or "Alto." If you want to create large jumps in pitch, select the option that least matches the range of the recorded audio. This tricks Autotune into correcting a note by moving it from one register to another.
Set the "Tracking" parameter. For a subtle effect, set it low. For an intense effect, set it high. The two extremes of this parameter are called "Relaxed" and "Choosy." The former configures Autotune to correct only very out-of-tune notes. The latter configures Autotune to correct any note that isn't perfectly in tune.