* Donkey - Shrek's talkative, sarcastic, and loyal companion, a brown talking donkey
* Princess Fiona - the beautiful, independent, and strong-willed princess, who is actually an ogre
* Lord Farquaad - the villain, a short, scheming, and cruel lord of Duloc, who wants to marry Fiona
* The Fairy Godmother - the scheming and manipulative queen of Duloc, who helps Farquaad in his quest to marry Fiona
* Prince Charming - the handsome, but arrogant and selfish prince, who is Fiona's fiancé
* Pinocchio - a wooden puppet who wants to be a real boy
* The Gingerbread Man - a gingerbread cookie who comes to life and joins Shrek and his friends
* The Big Bad Wolf - the villain from the fairy tale, who is now a member of Shrek's gang
* The Three Blind Mice - three blind mice who are friends of Shrek and Donkey
* Dragon - a giant, fire-breathing dragon who is Fiona's guardian
* The Muffin Man - a baker who makes delicious muffins
* The White Rabbit - a white rabbit who is the herald for Lord Farquaad
* The Magic Mirror - a magical mirror that shows Farquaad different princesses
* The Pied Piper - a musician who can control rats with his music
* The Three Little Pigs - three little pigs who are chased by the Big Bad Wolf
* The Ugly Duckling - a duckling who is shunned by the other ducks because of his appearance, but eventually turns into a beautiful swan