* Graham Chapman as Brian Cohen
* John Cleese as Reg
* Terry Gilliam as Various roles
* Eric Idle as Stan/Loretta
* Terry Jones as Mandy/Various roles
* Michael Palin as Francis/Pilate
* Carol Cleveland as Judith Iscariot
Supporting cast:
* Kenneth Colley as Jesus of Nazareth
* Charles McKeown as the Angel of the Lord
* Spike Milligan as the Prophet of Nazareth
* John Young as the Centurion
* Patsy Byrne as the Centurion's Wife
* Gwen Taylor as Judith Iscariot's Mother
* Neil Innes as the Innkeeper
* Victor Spinetti as the Innkeeper's Wife
* Bernard Bresslaw as the Jailer
* Terence Bayler as the Executioner
* David Warwick as the Assistant Executioner
* Christopher Biggins as the Leper
* Sue Jones-Davies as the Leper's Mother
* John Fortune as the Samaritan
* Carol Leader as the Samaritan's Wife
* Roger Watkins as the Wiseman
* Simon Jones as the Young Brian
* Andrew Sachs as the First Wise Man
* Roy Barraclough as the Second Wise Man
* Graham Crowden as the Third Wise Man