- Chris Pine as James T. Kirk
- Zachary Quinto as Spock
- Karl Urban as Leonard McCoy
- Zoe Saldana as Uhura
- John Cho as Hikaru Sulu
- Anton Yelchin as Pavel Chekov
- Simon Pegg as Montgomery Scott
- Bruce Greenwood as Christopher Pike
- Winona Ryder as Amanda Grayson
- Eric Bana as Nero