| Character | Actor |
|---|---|
| Jake Sully | Sam Worthington |
| Neytiri | Zoe Saldana |
| Colonel Miles Quaritch | Stephen Lang |
| Dr. Grace Augustine | Sigourney Weaver |
| Trudy Chacon | Michelle Rodriguez |
| Tsu'tey | Laz Alonso |
| Parker Selfridge | Giovanni Ribisi |
| Norm Spellman | Joel David Moore |
| Dr. Max Patel | Dileep Rao |
| Eytukan | Wes Studi |
| Mo'at | CCH Pounder |