- Annie, the title character, is a young orphan girl who is taken in by Oliver "Daddy" Warbucks.
- Oliver "Daddy" Warbucks, Annie's wealthy benefactor and adoptive father.
- Grace Farrell, Warbucks' secretary and a mother figure to Annie.
- Sandy, Annie's stray dog.
Secondary Characters
- Miss Agatha Hannigan, the cruel and alcoholic owner of the orphanage where Annie lives.
- Rooster Hannigan, Miss Hannigan's brother and a con man.
- Lily St. Regis, a scheming woman who poses as Annie's mother in an attempt to collect Warbucks' fortune.
- Bert Healy, a radio host and Annie's ally.
- Punjab, Warbucks' loyal valet.
- The Bundles, a group of orphans who live at the orphanage with Annie.
Villains
- Harold Gray, a crooked stockbroker who works with Lily St. Regis to swindle Warbucks.
- The Asps, a secret society of criminals who are led by Gray.