- Manny the mammoth (voiced by Ray Romano)
- Sid the sloth (voiced by John Leguizamo)
- Diego the saber-tooth tiger (voiced by Denis Leary)
- Ellie the female mammoth (voiced by Queen Latifah)
- Crash and Eddie the opossums (voiced by Seann William Scott and Josh Peck)
- Scrat the saber-tooth squirrel (voiced by Chris Wedge)
- Carlos the vulture (voiced by Carlos Saldanha)
- Gupta the vulture (voiced by Aziz Ansari)
- Raz the saber-tooth tiger (voiced by TJ Miller)