Mary Jane Watson: She is Spider-Man's longtime love interest and often considered his "main" heroine. She is a strong, independent woman who has faced her own challenges and supported Peter throughout his life.
Gwen Stacy: Another significant love interest and a superhero in her own right, known as "Ghost-Spider." Her tragic death in the comics is a pivotal moment in Spider-Man's story.
Aunt May: Peter's loving and supportive aunt, who acts as a mother figure. She is a source of strength and guidance, even in difficult situations.
Black Cat: A cunning and seductive anti-heroine with a complicated relationship with Spider-Man. She is known for her agility and thievery, but she also occasionally works with Spider-Man to combat crime.
Spider-Gwen: An alternate universe version of Gwen Stacy who takes on the mantle of Spider-Woman. She is a powerful hero with a distinct personality and backstory.
Silk: A young woman who gained spider-like abilities from the same radioactive spider that bit Peter. She is a skilled fighter and a strong independent hero.
So, depending on what you consider a "heroine," there are many different female characters in Spider-Man who could be considered. Ultimately, it's up to you to decide who you consider the most important.