Load the CD containing your intended guitar sample into your computer. When prompted, select "Import Audio."
Open your preferred music production software program, for example Cubase, Pro Tools or Logic. You can do this by clicking on the desktop icon or selecting the program the "Start" menu if using PC, or the "Applications" folder if using a Mac.
Import the audio file. Select "File" and select "Import." Browse your computer for the audio file from which you want to take the guitar sample. Once imported, the audio file appears in the program interface as a graphic representation of the audio sound waves, with large peaks for loud parts and small peaks for quiet parts.
Play the audio and listen out for sections where the guitar is prominent. For example, in the song "Sunshine Of Your Love" by Cream, the guitar part is played solo in the first few bars. Once the drums and vocals kick in, the guitar is accompanied. Highlight the "cleanest" section with the least interference from other instruments. It may be that the guitar is never played solo, in which case select the section with least accompaniment. Once you identify a suitable section of audio, cue up the track three to five seconds before that section by scrolling the navigation bar to the left.
Open "Tools" and select the scissor tool. Click "View" and select "Zoom In." Hit "Play" and as soon as the guitar part happens, press "Pause." Identify the peak that represents the start of the section. It is typically the first large peak to the right of where the track counter bar stopped. Click the scissor tool to "snip" the audio file.
Hit "Play" and pause the track at the end of the section you want to separate. Click the "Scissor" tool to the right of the final sound wave peak.
Select the "Pointer" tool from the "Tools" menu. Right click on the audio track anywhere between the two "snips." Select "Copy."
Click "File" and select "New Track." Right-click in the track and select "Paste." This imports the guitar audio snippet into a new track.
Select "Equalizer" from the "Tools" menu. Adjust the various virtual sliders to boost the frequency range between 125Hz and 3kHz and cut the rest. By boosting the frequencies of the guitar and cutting all other frequencies, you effectively mute any other audio on the track.