- Horatio Caine's Wife, Marina, played by Sofia Milos: Marina Caim was Horatio's wife during the early seasons of the show. She was originally a private investigator who helped Horatio on some cases. However, their marriage eventually fell apart due to Horatio's dedication to his work.
- Calleigh Duquesne, played by Emily Procter: Calleigh Duquesne is a CSI Level 3 and the daughter of a Miami police officer. She is a skilled forensic scientist and is known for her expertise in DNA analysis and firearm identification.
- Natalia Boa Vista, played by Eva LaRue: Natalia Boa Vista joins the team in Season 5 as a CSI Level 2. She is a former undercover police officer and has a background in psychology, which helps her understand the motivations of criminals.
- Delko's Wife, Marisol Delko, played by Elizabeth Berkley Lauren: Marisol Delko is Eric Delko's wife. They get married in the season finale of Season 4. Marisol also works as a homicide detective in Miami.
- Yelina Salas, played by Sofia Vassilieva: Yelina Salas joins the team in Season 9. She is the daughter of a Russian mobster and has knowledge about the inner workings of criminal organizations.