Film:
* Walk Hard: The Dewey Cox Story (2007): Played "Barry"
* The Ten (2007): Played "Rabbi"
* The Other Guys (2010): Played "Man at the Beach"
* The Heat (2013): Played "DEA Agent"
* The Secret Life of Pets (2016): Voice of "Mel"
* The Secret Life of Pets 2 (2019): Voice of "Mel"
Television:
* Upright Citizens Brigade (TV series): Creator, writer, and performer (1998-2000)
* The UCB Show (TV series): Creator, writer, and performer (2000-2006)
* Late Night with Conan O'Brien: Frequent guest performer (1998-2009)
* The Office (US): Played "Kevin's Dad" (2009)
* Parks and Recreation: Played "Doug" (2012)
* Brooklyn Nine-Nine: Played "Captain Holt's Dad" (2013)
* Comedy Bang! Bang!: Guest performer (2012-2015)
* The League: Played "The Coach" (2015)
* Big Mouth (TV series): Voice of "Jay Bilzerian" (2017-present)
* The Good Place: Played "Shawn" (2019)
* The Afterparty: Played "Detective Danner" (2022)
He has also appeared in several other television series and films in smaller roles.
It's worth noting that Matt Besser's primary contribution to the entertainment industry is through his work with UCB. He has written, directed, and performed in numerous live and online sketches and shows, as well as co-founded the UCB Theatre in New York and Los Angeles.