- Itachi Uchiha (Anbu): Defeat him by clearing the third stage of Shippuuden's Main Story.
- Jiraiya (Part II): Defeat him by clearing the fourth stage of Shippuuden's Main Story.
- Kabuto (Sage): Defeat him by clearing the fifth stage of Shippuuden's Main Story.
- Sasuke (Part II): Defeat him by clearing the sixth stage of Shippuuden's Main Story.
- Naruto (Six Tails Chakra Mode): Defeat him by clearing the seventh stage of Shippuuden's Main Story.
2. DLC Characters:
- Haku (Part II)
- Kimimaro
- Zabuza