* William Kemp (c. 1550–1603): Kemp was a popular comedian who was also a member of the Lord Chamberlain's Men. He was known for his physical comedy and his ability to improvise. Kemp left the Lord Chamberlain's Men in 1599 to join a rival acting company, the Admiral's Men.
* Robert Armin (c. 1563–1615): Armin was a comedian who replaced William Kemp in the Lord Chamberlain's Men. He was known for his quick wit and his ability to create memorable characters. Armin was a member of the Lord Chamberlain's Men until 1608.
* Edward Alleyn (1566–1626): Alleyn was an actor and theatre manager who was a contemporary of Shakespeare. He was the founder of the Fortune Theatre, one of the most popular theatres in London during Shakespeare's time. Alleyn was also a member of the Lord Admiral's Men, the acting company that was Shakespeare's main rival.
* Thomas Pope (c. 1570–1604): Pope was an actor who was a member of the Lord Chamberlain's Men. He was known for his versatility and his ability to play a variety of roles. Pope died of the plague in 1604.
* John Heminges (c. 1556–1630): Heminges was an actor and theatre manager who was a member of the Lord Chamberlain's Men. He was one of the two actors who edited the First Folio of Shakespeare's plays.
* Henry Condell (c. 1562–1642): Condell was an actor and theatre manager who was a member of the Lord Chamberlain's Men. He was one of the two actors who edited the First Folio of Shakespeare's plays.