Current:
* Agent Special Agent Jessica Knight: Played by Katrina Law, introduced in Season 19.
* Agent Special Agent Vanessa "Vee" Parker: Played by Diona Reasonover, introduced in Season 19.
* Dr. Jacqueline "Jack" Sloane: Played by Maria Bello, a recurring character starting in Season 14 and main cast in Season 16 & 17.
Past:
* Special Agent Ziva David: Played by Cote de Pablo, a main cast member from Season 3-13 (and some appearances since).
* Special Agent Kate Todd: Played by Sasha Alexander, a main cast member in Seasons 1-2.
* Special Agent "Jenny" Shepard: Played by Lauren Holly, a main cast member in Season 4-6.
* Director Eleanor Bishop: Played by Emily Wickersham, a main cast member in Seasons 11-19.
* Dr. Palmer: Played by Pauley Perrette, a main cast member in Seasons 1-15.
There have been many other female characters who have appeared throughout the series, but these are the main characters that have had the most impact on the show.