Here's a breakdown:
* Initial Discomfort: Claudius is visibly disturbed by the play's content, particularly the scene mirroring his own murder of King Hamlet. He attempts to dismiss it as "a most foul and unnatural murder," but his words ring hollow.
* Growing Guilt: As the play progresses, Claudius's discomfort escalates into palpable anxiety. He fidgets, sweats, and tries to excuse himself, but Hamlet's manipulation forces him to stay.
* Confrontation: The climax of the play, where the king is poisoned and the queen laments, hits Claudius particularly hard. He exclaims, "Give me some light! Away!" and rushes out of the hall, unable to bear the guilt any longer.
Claudius's reaction reveals his inner turmoil and provides crucial evidence of his guilt. His attempts to control the situation and escape the play's impact only serve to expose his culpability further.
This reaction is significant because it serves as a turning point in the play, solidifying Hamlet's suspicions and setting the stage for further confrontation.