* Ken Berry as Andy Taylor (the role was previously played by Andy Griffith in "The Andy Griffith Show")
* Linda Kaye Henning as Thelma Lou (also appearing on "The Andy Griffith Show")
* Buddy Ebsen as Jed Clampett (played Jed Clampett in "Beverly Hillbillies")
* Paul Hartman as Emmett Clark
* Peggy Rea as Helen Crump (played Helen Crump in "The Andy Griffith Show")
* Howard McNear as Floyd the Barber (played Floyd the Barber in "The Andy Griffith Show")
* Frances Bavier as Aunt Bee (played Aunt Bee in "The Andy Griffith Show")
The show also featured recurring guest stars such as:
* Don Knotts as Barney Fife (appeared in several episodes)
* Jim Nabors as Gomer Pyle (appeared in several episodes)
Mayberry RFD was a spin-off of "The Andy Griffith Show" and ran for three seasons, from 1968 to 1971.