- The original Globe Theatre was built on the south bank of the River Thames, while the reconstructed theatre is located on the north bank.
- The original Globe Theatre had a thatched roof, while the reconstructed theatre has a tiled roof.
- The original Globe Theatre was a wooden structure, while the reconstructed theatre has a steel frame.
- The reconstructed theatre is slightly larger than the original Globe Theatre.
Despite these differences, the reconstructed Globe Theatre is generally considered to be a faithful reproduction of the original. It is used for performances of Shakespeare's plays and other Elizabethan dramas, and it is a popular tourist destination.