* Miss Hannigan: The mean and cruel orphanage matron, who treats the orphans under her care very badly.
* Grace Farrell: The wealthy woman who comes to the orphanage to adopt Annie. She is kind and generous, and she quickly falls in love with Annie.
* Oliver Warbucks: Grace Farrell's employer, a rich businessman who is also kind and generous. He eventually adopts Annie and gives her a new home.
* Rooster: Miss Hannigan's scheming brother, who tries to kidnap Annie so he can collect the reward money.
* Lily: Miss Hannigan's other brother, who is also involved in the kidnapping plot.
* Pepper: One of the orphans who lives at the orphanage. She is Annie's best friend, and she is always there for her.
* July: Another one of the orphans who lives at the orphanage. She is a kind and gentle girl, who is often picked on by the other orphans.
* Tessie: Another one of the orphans who lives at the orphanage. She is a tomboyish girl, who loves to play pranks on the other orphans.
* Kate: Another one of the orphans who lives at the orphanage. She is a shy and timid girl, who is often afraid to speak her mind.
* Molly: Another one of the orphans who lives at the orphanage. She is a kind and caring girl, who always looks out for the other orphans.