- The conflict between Beatrice and Benedick: Beatrice and Benedick have a long-standing rivalry and frequently engage in witty banter and quarrels. Their conflict is largely based on their pride and mutual dislike, and it prevents them from admitting their true feelings for each other.
- The conflict caused by Don Pedro's deception: Don Pedro, in an attempt to win Hero's affection, devises a plan to make Claudio believe that Hero has been unfaithful. This deception leads to Claudio's rejection of Hero at the altar and causes great distress and conflict within the community.
- The conflict between Don John and Claudio: Don John, the illegitimate brother of Don Pedro, is a villainous character who plots to create conflict and sow discord among the other characters. He manipulates Claudio into believing Hero's infidelity and also tries to sabotage the relationship between Claudio and Hero.
- The conflict between the Watchmen and the lawbreakers: The Watchmen, led by Dogberry and Verges, are responsible for maintaining law and order in the town, but they are often incompetent and bumbling. Their conflict with the lawbreakers, such as Borachio and Conrad, highlights the challenges of maintaining justice and the difficulties faced by those in authority.
- The conflict between Hero and Claudio: Hero and Claudio are initially in love and engaged to be married. However, Claudio is misled by Don Pedro's deception and publicly accuses Hero of infidelity, leading to their separation and conflict.