* The appearance of Banquo's ghost in Act 2, Scene 1: The ghost of Banquo appears to Macbeth and accuses him of murder, causing Macbeth to become even more guilt-ridden and paranoid.
* Macbeth's conversation with Lady Macbeth in Act 2, Scene 2: Lady Macbeth tries to convince Macbeth to move on from the murder of Duncan, but Macbeth is unable to do so. He is consumed by guilt and fear, and he begins to spiral out of control.
* The news of Macduff's escape in Act 2, Scene 4: Macbeth learns that Macduff has fled to England, and he realizes that his grip on power is slipping. He becomes even more desperate and ruthless, and he is willing to do anything to maintain his position as king.