- Ice Cube as Calvin Palmer, the owner of the barbershop
- Cedric the Entertainer as Eddie, a barber at Calvin's shop
- Eve as Terri, a single mother and love interest of Calvin
- Troy Garity as Isaac, a white man living in the predominantly black neighborhood where the barbershop is located
- Sean Patrick Thomas as Jimmy James, a boxer and love interest of Terri
- Michael Ealy as Ricky Nash, a young man who works at the barbershop
- Harry Lennix as Lester Wallace, a local pastor
- Jazsmin Lewis as Draya, Terri's daughter
- Leonard Earl Howze as Dinka, a regular customer at the barbershop
- Lahmard J. Tate as J.D., a barber at Calvin's shop
- De'Angelo Wilson as Wideload, a barber at Calvin's shop
- Anthony Anderson as J.D.'s cousin
- Kevin Hart as Jimmy's sparring partner
- Jenifer Lewis as Ms. Doris, Terri's mother