* Bugs Bunny: The leader and most recognizable member of the Tune Squad.
* Daffy Duck: The greedy, ego-driven duck who often acts as a foil to Bugs.
* Lola Bunny: A tough and athletic rabbit, introduced specifically for Space Jam.
* Tweety Bird: The tiny, seemingly helpless bird with a surprising amount of sass.
* Tasmanian Devil: The whirlwind of destruction, always a force to be reckoned with.
* Porky Pig: The stutter-prone pig with a heart of gold.
* Road Runner: The speedy bird constantly running away from Wile E. Coyote.
* Wile E. Coyote: The hapless coyote who is always trying to catch the Road Runner.
* Michigan J. Frog: The crooner who often gets in trouble for his love of singing.
* Marvin the Martian: The extraterrestrial genius who plots against Earth.
Important note: While all these characters were part of the Tune Squad, only Bugs, Daffy, Lola, Tweety, and Taz actually played in the basketball game against the Monstars.