* Nina Tucker: Nina Tucker is the daughter of Shou Tucker, a State Alchemist. She is merged with her dog, Alexander, in a failed experiment.
* Barry the Chopper: Barry the Chopper is a Homunculus who is killed by Greed.
* Lust: Lust is a Homunculus who is killed by Mustang and Ed.
* Gluttony: Gluttony is a Homunculus who is killed by Ed and Al.
* Envy: Envy is a Homunculus who is killed by Mustang.
* Dante: Dante is the creator of the Homunculi. She is killed by Hohenheim.
* Hohenheim: Hohenheim is Ed and Al's father. He is killed by Father, the final Homunculus.
* Father: Father is the final Homunculus. He is killed by Ed and Al.